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Necroptosis-associated genes were induced in early-stage brain homogenates during B1.617.2 infection. The brain homogenates obtained at 3 and 4 dpi were analyzed simultaneously to profile 84 necrosis genes by using the <t>RT</t> <t>2</t> profiler <t>PCR</t> assay. The heat maps present the upregulated genes in the brain homogenates (left panel), and a scatter plot reveals the upregulated (red), downregulated (blue), and unchanged (black) genes ( A ). The plot presents the log tenfold changes in gene expression in the two groups. The quantification of the cell death-related genes Bcl2 (pro-apoptotic), Ripk3 (necroptosis), Casp3 (apoptotic), and Becn1 (autophagic) in the brain homogenates at the indicated time points after infection ( B ). Based on the GeneGlobe database ( https://dataanalysis2.qiagen.com/pcr ), the heat map for the RT 2 -profiler PCR array was generated using GraphPad Prism 7.0 ( https://www.graphpad.com/ ).
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Necroptosis-associated genes were induced in early-stage brain homogenates during B1.617.2 infection. The brain homogenates obtained at 3 and 4 dpi were analyzed simultaneously to profile 84 necrosis genes by using the <t>RT</t> <t>2</t> profiler <t>PCR</t> assay. The heat maps present the upregulated genes in the brain homogenates (left panel), and a scatter plot reveals the upregulated (red), downregulated (blue), and unchanged (black) genes ( A ). The plot presents the log tenfold changes in gene expression in the two groups. The quantification of the cell death-related genes Bcl2 (pro-apoptotic), Ripk3 (necroptosis), Casp3 (apoptotic), and Becn1 (autophagic) in the brain homogenates at the indicated time points after infection ( B ). Based on the GeneGlobe database ( https://dataanalysis2.qiagen.com/pcr ), the heat map for the RT 2 -profiler PCR array was generated using GraphPad Prism 7.0 ( https://www.graphpad.com/ ).
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Necroptosis-associated genes were induced in early-stage brain homogenates during B1.617.2 infection. The brain homogenates obtained at 3 and 4 dpi were analyzed simultaneously to profile 84 necrosis genes by using the RT 2 profiler PCR assay. The heat maps present the upregulated genes in the brain homogenates (left panel), and a scatter plot reveals the upregulated (red), downregulated (blue), and unchanged (black) genes ( A ). The plot presents the log tenfold changes in gene expression in the two groups. The quantification of the cell death-related genes Bcl2 (pro-apoptotic), Ripk3 (necroptosis), Casp3 (apoptotic), and Becn1 (autophagic) in the brain homogenates at the indicated time points after infection ( B ). Based on the GeneGlobe database ( https://dataanalysis2.qiagen.com/pcr ), the heat map for the RT 2 -profiler PCR array was generated using GraphPad Prism 7.0 ( https://www.graphpad.com/ ).

Journal: Scientific Reports

Article Title: Delta (B1.617.2) variant of SARS-CoV-2 induces severe neurotropic patterns in K18-hACE2 mice

doi: 10.1038/s41598-023-29909-x

Figure Lengend Snippet: Necroptosis-associated genes were induced in early-stage brain homogenates during B1.617.2 infection. The brain homogenates obtained at 3 and 4 dpi were analyzed simultaneously to profile 84 necrosis genes by using the RT 2 profiler PCR assay. The heat maps present the upregulated genes in the brain homogenates (left panel), and a scatter plot reveals the upregulated (red), downregulated (blue), and unchanged (black) genes ( A ). The plot presents the log tenfold changes in gene expression in the two groups. The quantification of the cell death-related genes Bcl2 (pro-apoptotic), Ripk3 (necroptosis), Casp3 (apoptotic), and Becn1 (autophagic) in the brain homogenates at the indicated time points after infection ( B ). Based on the GeneGlobe database ( https://dataanalysis2.qiagen.com/pcr ), the heat map for the RT 2 -profiler PCR array was generated using GraphPad Prism 7.0 ( https://www.graphpad.com/ ).

Article Snippet: Based on the GeneGlobe database ( https://dataanalysis2.qiagen.com/pcr ), the heat map for the RT 2 -profiler PCR array was generated using GraphPad Prism 7.0 ( https://www.graphpad.com/ ).

Techniques: Infection, Expressing, Generated